Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase.
Identifieur interne : 001304 ( Main/Exploration ); précédent : 001303; suivant : 001305Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase.
Auteurs : Jin-Beom Cho [Corée du Sud] ; Jin-Moo Lee [Corée du Sud] ; Hee-Chul Ahn [Corée du Sud] ; Yong-Joo Jeong [Corée du Sud]Source :
- Journal of microbiology and biotechnology [ 1738-8872 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- antagonistes et inhibiteurs : Helicase.
- enzymologie : Virus du SRAS.
- isolement et purification : Antienzymes, Antiviraux.
- métabolisme : Adénosine triphosphate.
- pharmacologie : Antienzymes, Antiviraux.
- Concentration inhibitrice 50, Hydrolyse.
English descriptors
- KwdEn :
- Adenosine Triphosphate (metabolism), Antiviral Agents (isolation & purification), Antiviral Agents (pharmacology), DNA Helicases (antagonists & inhibitors), Enzyme Inhibitors (isolation & purification), Enzyme Inhibitors (pharmacology), Hydrolysis, Inhibitory Concentration 50, SARS Virus (enzymology).
- MESH :
- chemical , antagonists & inhibitors : DNA Helicases.
- chemical , isolation & purification : Antiviral Agents, Enzyme Inhibitors.
- chemical , metabolism : Adenosine Triphosphate.
- chemical , pharmacology : Antiviral Agents, Enzyme Inhibitors.
- enzymology : SARS Virus.
- Hydrolysis, Inhibitory Concentration 50.
Abstract
A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC50 values of 8.66 ± 0.26 μM and 41.6 ± 2.3 μM, respectively. Moreover, we observed that the compound did not show cytotoxicity up to 80 µMconcentration. Our results suggest that the compound might serve as a SARS coronavirus inhibitor.
DOI: 10.4014/jmb.1507.07078
PubMed: 26387819
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000D63
- to stream PubMed, to step Curation: 000D63
- to stream PubMed, to step Checkpoint: 000E07
- to stream Ncbi, to step Merge: 002B34
- to stream Ncbi, to step Curation: 002B34
- to stream Ncbi, to step Checkpoint: 002B34
- to stream Main, to step Merge: 001306
- to stream Main, to step Curation: 001304
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase.</title><author><name sortKey="Cho, Jin Beom" sort="Cho, Jin Beom" uniqKey="Cho J" first="Jin-Beom" last="Cho">Jin-Beom Cho</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Lee, Jin Moo" sort="Lee, Jin Moo" uniqKey="Lee J" first="Jin-Moo" last="Lee">Jin-Moo Lee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Ahn, Hee Chul" sort="Ahn, Hee Chul" uniqKey="Ahn H" first="Hee-Chul" last="Ahn">Hee-Chul Ahn</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmacy, Dongguk University-Seoul, Goyang 410-820, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Pharmacy, Dongguk University-Seoul, Goyang 410-820</wicri:regionArea><wicri:noRegion>Goyang 410-820</wicri:noRegion></affiliation></author><author><name sortKey="Jeong, Yong Joo" sort="Jeong, Yong Joo" uniqKey="Jeong Y" first="Yong-Joo" last="Jeong">Yong-Joo Jeong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="RBID">pubmed:26387819</idno><idno type="pmid">26387819</idno><idno type="doi">10.4014/jmb.1507.07078</idno><idno type="wicri:Area/PubMed/Corpus">000D63</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000D63</idno><idno type="wicri:Area/PubMed/Curation">000D63</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000D63</idno><idno type="wicri:Area/PubMed/Checkpoint">000E07</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000E07</idno><idno type="wicri:Area/Ncbi/Merge">002B34</idno><idno type="wicri:Area/Ncbi/Curation">002B34</idno><idno type="wicri:Area/Ncbi/Checkpoint">002B34</idno><idno type="wicri:Area/Main/Merge">001306</idno><idno type="wicri:Area/Main/Curation">001304</idno><idno type="wicri:Area/Main/Exploration">001304</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase.</title><author><name sortKey="Cho, Jin Beom" sort="Cho, Jin Beom" uniqKey="Cho J" first="Jin-Beom" last="Cho">Jin-Beom Cho</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Lee, Jin Moo" sort="Lee, Jin Moo" uniqKey="Lee J" first="Jin-Moo" last="Lee">Jin-Moo Lee</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author><author><name sortKey="Ahn, Hee Chul" sort="Ahn, Hee Chul" uniqKey="Ahn H" first="Hee-Chul" last="Ahn">Hee-Chul Ahn</name><affiliation wicri:level="1"><nlm:affiliation>Department of Pharmacy, Dongguk University-Seoul, Goyang 410-820, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Pharmacy, Dongguk University-Seoul, Goyang 410-820</wicri:regionArea><wicri:noRegion>Goyang 410-820</wicri:noRegion></affiliation></author><author><name sortKey="Jeong, Yong Joo" sort="Jeong, Yong Joo" uniqKey="Jeong Y" first="Yong-Joo" last="Jeong">Yong-Joo Jeong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702, Republic of Korea.</nlm:affiliation><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Bio and Nanochemistry, Kookmin University, Seoul 136-702</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName></affiliation></author></analytic><series><title level="j">Journal of microbiology and biotechnology</title><idno type="eISSN">1738-8872</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenosine Triphosphate (metabolism)</term><term>Antiviral Agents (isolation & purification)</term><term>Antiviral Agents (pharmacology)</term><term>DNA Helicases (antagonists & inhibitors)</term><term>Enzyme Inhibitors (isolation & purification)</term><term>Enzyme Inhibitors (pharmacology)</term><term>Hydrolysis</term><term>Inhibitory Concentration 50</term><term>SARS Virus (enzymology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adénosine triphosphate (métabolisme)</term><term>Antienzymes (isolement et purification)</term><term>Antienzymes (pharmacologie)</term><term>Antiviraux (isolement et purification)</term><term>Antiviraux (pharmacologie)</term><term>Concentration inhibitrice 50</term><term>Helicase (antagonistes et inhibiteurs)</term><term>Hydrolyse</term><term>Virus du SRAS (enzymologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>DNA Helicases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antiviral Agents</term><term>Enzyme Inhibitors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Adenosine Triphosphate</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Enzyme Inhibitors</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Helicase</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antienzymes</term><term>Antiviraux</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adénosine triphosphate</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antienzymes</term><term>Antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Hydrolysis</term><term>Inhibitory Concentration 50</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Concentration inhibitrice 50</term><term>Hydrolyse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A new chemical inhibitor against severe acute respiratory syndrome (SARS) coronavirus helicase, 7-ethyl-8-mercapto-3-methyl-3,7-dihydro-1H-purine-2,6-dione, was identified. We investigated the inhibitory effect of the compound by conducting colorimetry-based ATP hydrolysis assay and fluorescence resonance energy transfer-based double-stranded DNA unwinding assay. The compound suppressed both ATP hydrolysis and double-stranded DNA unwinding activities of helicase with IC50 values of 8.66 ± 0.26 μM and 41.6 ± 2.3 μM, respectively. Moreover, we observed that the compound did not show cytotoxicity up to 80 µMconcentration. Our results suggest that the compound might serve as a SARS coronavirus inhibitor. </div></front></TEI><affiliations><list><country><li>Corée du Sud</li></country><region><li>Région capitale de Séoul</li></region><settlement><li>Séoul</li></settlement></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Cho, Jin Beom" sort="Cho, Jin Beom" uniqKey="Cho J" first="Jin-Beom" last="Cho">Jin-Beom Cho</name></region><name sortKey="Ahn, Hee Chul" sort="Ahn, Hee Chul" uniqKey="Ahn H" first="Hee-Chul" last="Ahn">Hee-Chul Ahn</name><name sortKey="Jeong, Yong Joo" sort="Jeong, Yong Joo" uniqKey="Jeong Y" first="Yong-Joo" last="Jeong">Yong-Joo Jeong</name><name sortKey="Lee, Jin Moo" sort="Lee, Jin Moo" uniqKey="Lee J" first="Jin-Moo" last="Lee">Jin-Moo Lee</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001304 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001304 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:26387819
|texte= Identification of a Novel Small Molecule Inhibitor Against SARS Coronavirus Helicase.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:26387819" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |